Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQILS 2ELH Chain:A ((21-67)) ---------------------------------------------------------------------------------------------RSLTPRDKIHAIQRIHDGESKASVARDI------GVPESTLRGWCKNEDKLRF------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ELH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 114 -14605 -128.11 -310.73 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -128.11 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.663

(partial model without unconserved sides chains): PDB file : Tito_2ELH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ELH-query.scw PDB file : Tito_Scwrl_2ELH.pdb: