Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELP---------EEFKSIVLSISKELELGVFPKVFVQKF---SPEVLGPPPSLVFFTDE--FETIYENMEEPG-E----ITDNNGILTFNFSDPEGNYFVIGKAESSD 3KOL Chain:A ((7-153)) --------SVNSVLAPGNLRKVHHIALNVQDMQASRYFYGTILGLHELTDDEVPATLTELVASGKVANFITPD--GTILDLFGEPELSPPDPNPEKTFTRAYHLAFDIDPQLFDRAVTVIGENKIAIAHGPVTR-TGRGVYFYDPDGFMIEIRCDPE--

General information: TITO was launched using: RESULT: Template: 3KOL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 324 -9442 -29.14 -74.35 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -29.14 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_3KOL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KOL-query.scw PDB file : Tito_Scwrl_3KOL.pdb: