Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKEITFSVFFLQFIEKSLYFASYSFFVTIRNGTSKKMKKQGTRYMEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKITEKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQLIDWSDPTQISHVFFHSLIVDPQKA-FHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLVNLNDLIQKDE-----QGNL--SFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEIKNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQYGDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAPILGK-TNILIYPFGADISDWQPYSAENQKFAYLKSQGFDI-FCNVDASTPAWGQLGTDFYRNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA 5JP6 Chain:A ((85-306)) -------------------------------------------------------------------------------------------------------------------------------------------------------LPAQDLTLFENEIRDENNRALVSG---CKEELLAQVDEHFDEQRESMSVPGHALKAVQSRNSFRFPDNTQKRDMSNGYMAVRGDVARKEVVLTFDD----------GPHGLYTD---------------------AILRALKEV----------NAKAMFFATGKSVR---------------------TNPEALKRVAADGHVIGSHSIT------LTFDEAAAEVRGGHQAVFDVLGWVDPVFRF-YGE-------TS--KDLKAFLKTKSTGEFAWNIESD-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5JP6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 734 29148 39.71 151.03 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 39.71 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_5JP6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JP6-query.scw PDB file : Tito_Scwrl_5JP6.pdb: