Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYKKKSRNFSKNRGRSMNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEATRIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTKPIDVNEMILRVEALLRRS-KINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLYKLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGYKAVI 5ED4 Chain:E ((25-244)) -------------------RVLVVDDEANIVELLSVSLKFQGFEVYTATNGAQALDRARETRPDAVILDVMMPGMDGFGVLRRLRADGIDAPALFLTARDSLQDKIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG---RNVRLTFADIELDEETHEVWKAGQPVSLSPTEFTLLRYFVINAGTVLSKPKILDHVWRYDFGGDVNVVESYVSYLRRKIDTGEKRLLHTLRGVGY----

General information: TITO was launched using: RESULT: Template: 5ED4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 934 -84753 -90.74 -397.90 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain E : 0.79 3D Compatibility (PKB) : -90.74 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_5ED4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ED4-query.scw PDB file : Tito_Scwrl_5ED4.pdb: