Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAAKEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNADSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSDAVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQLILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGYDNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTAPLK 4RK0 Chain:C ((7-274)) -----------------------------------------------------------------SKTIGVIVPDITNPFFAQLIRGIESVLYKENFILILCNADQDVTREHEYLTELIRRSVDGFVIASSEISNQTINETLRAKKIPFIVLDQKKAEGFSDAVLTDDYRGGQLAAKHLQEQRHEQVIVVMPPHAPVNIQQRLKGFCS---VYT---EKVQLIETELSKTGGYQAVPEILKTESTGIFAINDEIAFGLYRGLAEAGKKIPEDYSIIGYDNVDMCEYVSPPLTTIAQPVFQLGQTTATLLLERIHQPAKDWEEQTLPVQLIERFSTAPLK

General information: TITO was launched using: RESULT: Template: 4RK0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1545 -153340 -99.25 -572.16 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -99.25 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_4RK0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RK0-query.scw PDB file : Tito_Scwrl_4RK0.pdb: