Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKKMIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQKQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTSYKLLSEK 1WWW Chain:X ((282-382)) VSFPASVQLHTAVEMHHWCIPFSVD-------GQPAPSLRWLFNGSVLNETSFIFTEFLEPAANETVRHGCLRLNQ---PTHVNNGNYTLLAANPFG--QASASIMAAFMDNP

General information: TITO was launched using: RESULT: Template: 1WWW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 459 -41614 -90.66 -412.02 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain X : 0.65 3D Compatibility (PKB) : -90.66 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.207

(partial model without unconserved sides chains): PDB file : Tito_1WWW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WWW-query.scw PDB file : Tito_Scwrl_1WWW.pdb: