Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAISTVNGIASLAGFEP-IVEMNE-EEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAENSEYIQIKTINDK 2IDL Chain:B ((4-116)) MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINFINSIEKFAGY-EPILELNEDEG-GYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENYSEFVQTRVITE-

General information: TITO was launched using: RESULT: Template: 2IDL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 459 -27118 -59.08 -244.30 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -59.08 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_2IDL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IDL-query.scw PDB file : Tito_Scwrl_2IDL.pdb: