Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGINIENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPDMPVTLVGDNGVTKATTVRALLPYAFTEKDL 1UX0 Chain:B ((3-130)) ----RQELITEALKARDMAYAPYSKFQVGAALLTKDGKVYRGCNIENAAYSMCNCAEQTALFKAVSEGDTEFQMLAVAADTPGPVSPCGACRQVISELCTKDVIVVLTNLQGQIKEMTVEELLPGAFSSEDL

General information: TITO was launched using: RESULT: Template: 1UX0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 658 -77978 -118.51 -609.20 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -118.51 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_1UX0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UX0-query.scw PDB file : Tito_Scwrl_1UX0.pdb: