Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKS-VMVMSEHYLEDIRLR-LSAEIGRGLT-LLEAKGGYNRKSKEVLLI-VV-TDHEFSRMKPLIEEIDPSAF-VIVSSVAEVMGRGFTYHPIE 3HLU Chain:A ((3-74)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DQQTMVYIVSAK-RKIIADRMLQ-ELDLGVTML-QAVGAYKNNETEVI--MCVMRKATLVKVRNLLKEVDPDAFMIVS-----------------

General information: TITO was launched using: RESULT: Template: 3HLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 198 2166 10.94 32.82 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.43 3D Compatibility (PKB) : 10.94 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_3HLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HLU-query.scw PDB file : Tito_Scwrl_3HLU.pdb: