Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQISGKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFSKRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINEILTELFAKVGLVLVDFKLEFG-RSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDLGDLIPVYQEVYNRLSELTEEKERV 4NYE Chain:B ((23-254)) ---KQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAGKGVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSKRFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINELLKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGN-HMDKDVFRRGLGELTDVYEIVWEKLQEL-------

General information: TITO was launched using: RESULT: Template: 4NYE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1145 -29467 -25.74 -127.56 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.88 3D Compatibility (PKB) : -25.74 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_4NYE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NYE-query.scw PDB file : Tito_Scwrl_4NYE.pdb: