Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFFQGHFPEEPVMPGVLIVEAMAQAGA-VALLSLEQF-----KGKTAYFGGLDKAKFRKKVTPGDTLYLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG 3ED0 Chain:E ((14-156)) --IEHILQILPHRYPMLLVDRITELQANQKIVAYKNITFNEDVFNGHFPNKPIFPGVLIVEGMAQSGGFLAFTSLWGFDPEIAKTKIVYFMTIDKVKFRIPVTPGDRLEYHLEVLKHKGMIWQVGGTAQVDGKVVAEAELKAMIA

General information: TITO was launched using: RESULT: Template: 3ED0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 622 -44315 -71.25 -323.47 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain E : 0.84 3D Compatibility (PKB) : -71.25 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_3ED0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ED0-query.scw PDB file : Tito_Scwrl_3ED0.pdb: