Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYGNELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNRMTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLLLILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFV-PGVFAK----IKGLEFLTEWFTETWLMYTDFA-----QQATY-------------------SQAPKMILVSLVTIVVSSALGMFIFQKSDIK 5DO7 Chain:A ((369-647)) -GVLLRRVTRNLVRNKLAVITRLLQNLIMGLFLLFFVLRVRS-N-V-LKG-AIQ-----DRVGLLYQFVGATPYTGMLNAVNLFPVLRAVSDQESQDGLYQKWQMMLAYALHVLPFSVVATMIFSSVCYW----TLGLH-PE-----VARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFKIIS--YFTFQKYCSEILVVNEFYGLNFTCAFTQGIQFIEKTCPGATSRFTMNFLILYSFIPALVILGIVVFKIRDH-

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1095 -172563 -157.59 -728.11 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -157.59 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: