Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLKAIKESLPEA--ELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHRDEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAVRAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIASAVAFAIETPDRMSVSNMIIRPTTQEV 3TFO Chain:B ((4-238)) -----DKVILITGASGGIGEGIARELGVAGAKILLGARRQARIEAIATEIRDAGGTALAQVLDVTDRHSVAAFAQAAVDTWGRIDVLVNNAGVMPLSPLAAVKVDEWERMIDVNIKGVLWGIGAVLPIMEAQRSGQIINIGSIGALSVVPTAAVYCATKFAVRAISDGLRQE--STNIRVTCVNPGVV-----------------------IALQPADIARAVRQVIEAPQSVDTTEITIRPT----

General information: TITO was launched using: RESULT: Template: 3TFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1160 -62379 -53.77 -295.63 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -53.77 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_3TFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TFO-query.scw PDB file : Tito_Scwrl_3TFO.pdb: