Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFDELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY 5J73 Chain:A ((6-80)) ---------------------------------------------------TSDYIIEQIQRDQEEAR--KKVEEAEERLERVKEASKRGVSSDQLLDLIRELAEIIEELIRIIRRSNEAIKELIKNQ-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5J73.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 106 -1794 -16.92 -23.91 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.41 3D Compatibility (PKB) : -16.92 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.41 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_5J73.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J73-query.scw PDB file : Tito_Scwrl_5J73.pdb: