Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCTAITYATKD--HYFGRNFDYEMSYNEVVTITPRNYR-FDFRKVKNLDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADY-KELQEGKDNVAPFEFIPWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLAD-KEKSIVIESMKDGLHLYDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTRV 2BJG Chain:B ((2-192)) -CTGLALETKDGLHLFGRNMDIEYSFNQSIIFIPRNFKCVNKSNKKELTTKYAVLGMGTIFDDYPTFADGMNEKGLGCAGLNFPVYVSYSKEDIEGKTNIPVYNFLLWVLANFSSVEEVKEALKNANIVDIPISENIPNTTLHWMISDITGKSIVVEQTKEKLNVFDNNIGVLTNSPTFDWHVANLNQYVGL------

General information: TITO was launched using: RESULT: Template: 2BJG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 992 31831 32.09 171.13 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : 32.09 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_2BJG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BJG-query.scw PDB file : Tito_Scwrl_2BJG.pdb: