Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTSGFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHE--EVPRDEKRGQFARASWGIDYHDILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLITEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSYQTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPMLSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECL-TDVRPVIRGTAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK 5D0B Chain:B ((34-372)) --LKEELIEYAKSIGVDKIGFTTADTFDSLKDRLILQESLGYLSGFEEPDIEKRVTPKLLLPKAKSIVAIALAYPSRMKDAPRSTRTERRGIFCRASWGKDYHDVLREKLDLLEDFLKS------KH-DIRTKSMVDTGELSDRAVAERAGIGFSAKNCMITTPEYGSYVYLAEMITNIPFEPDVPIEDMCGSCTKCLDACPTGALVNPGQLNAQRCISFLTQTKGFLPDEFRTKIGNRLYGCDTCQTVCPLNKGKDFHLHPEMEPDPEIAKPLLKPLLAISNREFKEKFGHVSGSWRGKKPIQRNAILALAHFKDASALPELTELMHKDPRPVIRGTAAWAIGKIG---------------------------------------

General information: TITO was launched using: RESULT: Template: 5D0B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1761 14557 8.27 43.45 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : 8.27 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_5D0B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D0B-query.scw PDB file : Tito_Scwrl_5D0B.pdb: