Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNYLDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV----------QNPGKEYYEQHM-----IGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSVITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQDLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLTLFGYWCYRKFKRQKQRKKNNR 2W1J Chain:B ((4-197)) ------------------------------------------------------------------QQIADFDKEKATLDEADIDERM-KLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHERMGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKMFTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYMINTHRLLVRGHRIPYV---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2W1J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 876 16349 18.66 91.33 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : 18.66 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_2W1J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W1J-query.scw PDB file : Tito_Scwrl_2W1J.pdb: