Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNEKKAVMITKKAGEDMLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVFHPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEETGYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQQAMKDQLICDAKTIYAIQYWELLTKGK 5C8L Chain:B ((3-166)) ----------------------HLEEKTLSTRQIFKGRYLKIEQDQVQAPDGRTYTREYILHPGAAMMIPLLPNGNVVMIHQYRHAVKKVFLEFPAGKRDHNE--ETLLTAKRELLEETGYEAKDWKFLTTIHPVIGYSNEHIDLYLARDLTHLEQRL--DQGQFIEVVEVKPADLMQLVLEGKVSDVKT--------------

General information: TITO was launched using: RESULT: Template: 5C8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 666 -35630 -53.50 -217.25 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -53.50 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_5C8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C8L-query.scw PDB file : Tito_Scwrl_5C8L.pdb: