Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGIT-IDHTFNEQLKGVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLDSLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVGLKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWHDHG 1Z4N Chain:B ((1-216)) MFKAVLFDLDGVITDTAEYHFRAWKALAEEIGINGVDRQFNEQLKGVSREDSLQKILDLADKK--VSAEEFKELAKRKNDNYVKMIQDVSPADVYPG------------IKIALASASKNGPFLLERMNLTGYFDAIADPAEVAASKPAPDIFIAAAHAVGVAPSESIGLEDSQAGIQAIKDSGALPIGVGRPEDLGDDIVIVPDTSHYTLEFLKEVW----

General information: TITO was launched using: RESULT: Template: 1Z4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1026 20807 20.28 102.50 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.90 3D Compatibility (PKB) : 20.28 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_1Z4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z4N-query.scw PDB file : Tito_Scwrl_1Z4N.pdb: