Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYKPHLSRDQAIALLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQPWKGDPVEQLVRHFLIETGP------------------KGVKIKGCPSEPYFGSLSALVSQH 2EYZ Chain:A ((13-104)) WYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSE---------------NSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIG----DQEFDSLPALLEFY

General information: TITO was launched using: RESULT: Template: 2EYZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 272 -9551 -35.11 -129.06 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -35.11 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_2EYZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EYZ-query.scw PDB file : Tito_Scwrl_2EYZ.pdb: