Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVAAQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFLTHAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKT----------------KDKGTGFKNEITPYL-YNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLDGKHPVFGQVIGGMDVVDKIAKAEK---DEKDKPTTAITIDSIEVVKDYDFKS 4R3F Chain:A ((13-203)) ----------------------------------------------------------------------QPTGAVIIHTTQGDLKVELFAKQTPLTCRNFLQHSLDGYYDGTIFHRLVPGFIIQGGDPTGTGHGGESIYDGGAFSGDLDPWPMDQRKGHNAGPMGVNFKDEFHSRLKFNRRGLLGMANEGAPDTNGSQFFFTLG----------------------K--AEELNNKNTLFGRVAAGDTIYNLMKWGEAELIEGTERPQYPVKITNIEILLNPFP--

General information: TITO was launched using: RESULT: Template: 4R3F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 849 6008 7.08 35.13 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 7.08 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.623

(partial model without unconserved sides chains): PDB file : Tito_4R3F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R3F-query.scw PDB file : Tito_Scwrl_4R3F.pdb: