Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVAAQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFLTHAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPYL-YNIRGALAMANTG--QPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLDGKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS 2POE Chain:A ((15-177)) -----------------------------------------------------------------------YFQGVRIITNYGDLKFELFCSQCPKACKNFLALSASGYYKNTIFHKNIKGFIIQGGDPTGTGKGGESIYG---------RYFDDEIYPELKYDRRGILSMASKGAKKPNTNGSQFFITYS----------------------S--LPQLNGEYVIFGKLIDGFETLNTLENCPSDKSHKPIDEIIIKDIVIHSNP----

General information: TITO was launched using: RESULT: Template: 2POE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 848 -23881 -28.16 -150.19 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -28.16 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.693

(partial model without unconserved sides chains): PDB file : Tito_2POE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2POE-query.scw PDB file : Tito_Scwrl_2POE.pdb: