Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence TPPGSPSARLAGTSNARLAGISNTNPPGGAPRPRQQCFLSADLGPDVMGQPTLLEATPFVNDMFDFHGYRCQMGKDCSHVRCRGFPAAFKMTARNYPDGKGPLGTSAGDSFVPGPVVKVYPAPR 1L8C Chain:A ((1-95)) --ADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRAC-----------SLPHCRTMKNVLNHMTH-----CQAGKACQVAHCASSRQIISHWKN-CTRHDCP--VCLPLKNASDKR--------

General information: TITO was launched using: RESULT: Template: 1L8C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 246 -32869 -133.61 -345.99 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -133.61 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_1L8C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L8C-query.scw PDB file : Tito_Scwrl_1L8C.pdb: