Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence NEDCIVSLVPDGGS--FARKTLRSSPGQQHDLQWGKYAFTIRVENGCAGKKTAGTIPPAHFV--SVGRAASLKPTSILRDGDPRPPTAVYPPRQPPRTEGRRTRYG-------SSG--GAVTVGAR 1PLC Chain:? ((1-99)) ---IDVLLGADDGSLAFVPSEFSISPGE-----------KIVFKN------NAGFPHNIVFDEDSIPSGVDAS-----KISMSEEDLLNAKGETFEVALSNKGEYSFYCSPHQGAGMVGKVTVN--

General information: TITO was launched using: RESULT: Template: 1PLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 349 12168 34.87 141.49 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 34.87 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_1PLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PLC-query.scw PDB file : Tito_Scwrl_1PLC.pdb: