Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWLESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHI-GYSIRPSERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVERYWIEVANE 2R1I Chain:B ((23-161)) EVPRRATPADAATVAQMLHDFNTEFGAPTP---GTD--ELASRLSHLLA------------GEDVVVLL-AGEPPTGLAVLSFRPNVWYPGPVAILDELYVRPGRRGHRLGSALLAASCGLVRSRGGALLEINVDGEDTDARRFYEARGFTNTEPNG------------

General information: TITO was launched using: RESULT: Template: 2R1I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 550 -36063 -65.57 -261.32 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -65.57 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_2R1I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R1I-query.scw PDB file : Tito_Scwrl_2R1I.pdb: