Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKNNSRQFINGQRVAVNVPALESIAAKDYEPTGYVFSQATLEEVDQAAQAAHNAFLVYSQTTQEQRASFLEEIARQIEALGANLQEVASLETGLPLARLQGETGRVTGQLRLFAELLRRGDFYGARIDVALPERKPLPRVDLRQYKVGVGPVAVFGASNFPLAFSTAGGDTVAALAAGCPVVFKAHSGHMATAELVAEAIEKAIEVCGMPKGTFNMIFGGR--IGANLVEHSLIQAAGFTGSLEGGMALYNLAQSRPQPIPFFAEMSSVNPVVVLPEALHSRGEQIAQDTVASFNMGCGQFCTKPGLILGVKGPEFDRFITALAENTQKAVPQVMLNEGTLKSYRAGIDALINEAGFEIIASGQEPELVSQAKAHLFKADQSVLLSGNPKLQHEVFGPMSIVIAVEDEATLLKGIEALGGQLTATIIGDENELSKAGELLDLLTRKAGRVLFNGFPTGVEVSDAMVHGGPFPATSDARGTSVGTGAIERFLRPVCFQNTPQVLLPDALKDSNPLQITRLVNGQLTQAQI 1EZ0 Chain:B ((24-506)) ------------------------------------FPVHTEVEVNQAATAAAKVARDFRRLNNSKRASLLRTIASELEARSDDIIARAHLETALPEVRLTGEIARTANQLRLFADVVNSGSYHQAILDTPNPTRAPLPKPDIRRQQIALGPVAVFGASNFPLAFSAAGGDTASALAAGCPVIVKGHTAHPGTSQIVAECIEQALKQEQLPQAIFTLLQGNQRALGQALVSHPEIKAVGFTGSVGGGRALFNLAHERPEPIPFYGELGAINPTFIFPSAMRAKAD-LADQFVASMTMGCGQFCTKPGVVFALNTPETQAFIETAQSLIRQQSPSTLLTPGIRDSYQSQVVSRGSDDGIDVTFSQAESPCVASA---LF-VTSSENWRKHPAWEEEIFGPQSLIVVCENVADMLSLSEMLAGSLTATIHATEEDYPQVSQLIPRLEEIAGRLVFNGWPTGVEVGYAMVHGGPYPASTHSASTSVGAEAIHRWLRPVAYQALPESLLPDSLKAENPLEIARAVDGK------

General information: TITO was launched using: RESULT: Template: 1EZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2851 -63539 -22.29 -132.10 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -22.29 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_1EZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EZ0-query.scw PDB file : Tito_Scwrl_1EZ0.pdb: