Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIWSI---------LCLIWTLVAYVVAKRIYQKHPKLWLSPAISVPVLTIILMTIFGISY-QTYAEDTQWIVNLL--GPATVAFAIP----IYRYRETIRKKLGVLTIAIIVGM-----SVGVMTAYKMAQWFHFS------PEVTNSLMARSISTPFAMILAEDIHGS------------------------AALVSL-----FTVITGLVGMICGDAILAITKIRSNVANGAALGNAAHGFGTVRAQQRNSEEGVIASLTMVIAGILMVLIGPFAIHL----------WLLL-------------------------------- 5EQJ Chain:B ((4-376)) -NCFSGYKDLIKEGDLTLIWVSRDNIKPVRMHS--EEVFNTRYGSFPHKDII-----GKPYGSQIAIRTFAFVHVLQPTPELWTLSLPTQIVYTPDSSYIMQRLNCSPHSRVIEAGTGSGSFSHAFARSVGHLFSFEFHHIRYEQALEEFKEHGLIDDNVTITHRDVCQGGFLIKKGDTTSYEFGNNETAASLNANVVFLDLPAPWDAIPHLDSVISVDEKVGLCCFSPCIEQVDKTLDVLEKYGWTDVEMVEIQGRQYESRRQMVRSLNDALERLRDIKRHIKEGDSNYKWKEVTKMEAEIKSHTSYLTFAFKVVNRSRDDEKVNE

General information: TITO was launched using: RESULT: Template: 5EQJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1149 -66723 -58.07 -301.91 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -58.07 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.087

(partial model without unconserved sides chains): PDB file : Tito_5EQJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EQJ-query.scw PDB file : Tito_Scwrl_5EQJ.pdb: