Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLFDDTAVSKQLAQVRIVMVNTTLPANIGSALRAMKTMGLSKLVLVSPKTYPHPDIQALAAGAQDLLEQVEIVETLEEAIKDCHLVFGTSARSRTIPWPLLDVRPASKEAIKAAAQGQQIAIVFGREDRGLTNEELALANYHLTIPVNSDYGVLNVAQAIQVVCYELRMSVLEQKEVASDAGQMPLAQGQSMQWDE---PLVTQQQMEEFYPHLEKVLTEIEFLDPENPRLLPLRLRRLFGRIQLDRMEYHLLRGIFSRVQALTSGKWKKASSDKEDQSNA 4XBO Chain:B ((2-240)) ------------LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKP-------AAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAAN-TPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQE-----------N-------ETPYPLV--DDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN-----------------

General information: TITO was launched using: RESULT: Template: 4XBO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1075 -144856 -134.75 -652.50 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -134.75 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.623

(partial model without unconserved sides chains): PDB file : Tito_4XBO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XBO-query.scw PDB file : Tito_Scwrl_4XBO.pdb: