Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRFYSLYLHDDACKNRQNLSSCRTYSVSLEFESRRDNMSNENQKPTSPTEQAQSSDKVSPFLSSPLPQGTPQGQQQSLQQTLTDTPVSGSVPKYNLPRGAGNTGNVGETTHFGYSTVRTEDKAQKVAEVFHSVASKYDLMNDLMSFGIHRLWKRFAINM--SGVRRGQ----HVLDIAGGTGDLA-----KVFSREVGPQGHVVLSDINESMLNVGRDRLIDAGCT----NVDFVLANAETLEPFADNSFDLVTISFGLRNVTDKDAALASMFRVLKPGGRLLVLEFSKPVFEPFSKLYDLYSFTALPIMGKLVANDSESYKYLAESIRMHPDQRTLKGMMENAGFQNCDYHNLTGGIVAVHRGFKL 4OBW Chain:C ((23-257)) -----------------------------------------------------------------------------------------------------------------------------------SVANRYDLMNDVMSLGIHRLWKDHFINKLDAGKRPNSTTPLNFIDVAGGSGDIAFGLLDHAESKFGDTESTMDIVDINPDMLKEGEKRAMEQGKYFKDPRVRFLVSNGEKLEEIDSDSKDIYTVSFGIRNFTDIQKGLNTAYRVLKPGGIFYCLEFSK-IENPLMDFAYQQWAKVLPVMGSMIANDYDSYQYLVESIERFPDQETFKSMIEKAGFKSAGYESLTFGICAIHWGIKV

General information: TITO was launched using: RESULT: Template: 4OBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 992 41146 41.48 187.03 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : 41.48 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_4OBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OBW-query.scw PDB file : Tito_Scwrl_4OBW.pdb: