Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHCPFCNAADSKVIDSRLAAEGCQIRRRRECVSCGERFTTFESYEVVMPRVIKSNGKNEPFDEAKLRRSLMHALQKRPVTQEQIETVLSDIQLQIRRLGERDVKSRTIGEIVMQSLFALDHVAYVRFASVYQDFQDVEAFRRQIEQMQQREH 2K4N Chain:A ((1-111)) --------MNSEVIKEFLEDIG--------------------------------EDYIELENEIHLKPEVFYEVWKYVGEPELKTYVIEDEIVEPGEYDPPEMKYTNVKKVKIKKVY-FETLDNVRVVTDYSEFQKILKKRGTKLEHHHHHH

General information: TITO was launched using: RESULT: Template: 2K4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 352 -17547 -49.85 -158.08 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -49.85 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_2K4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2K4N-query.scw PDB file : Tito_Scwrl_2K4N.pdb: