Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALFNSTSWAKYSVIAASLAGVMMLGGCAKKEEHTTTTLNIGYQ--KYG---ILPILKARGDLEKALKDQGVQVKWVEFPAGPQLLEGLNVGSVSLGEAGEAPPIFAQAANPNLVYVANQPAAPKAEALLVQKD--SPIQSIKDLKGKRVALN-KGSNVHYLLLKVLEANHLSLSDIQPVYLP-PSDARAAFEKEAVDAWVIWDPFFAAAEHQIQARVLATGE---------NLVSNHQFYLADRKFAENNPKVLNTVVQTLNQTTQWVSTH-QDEAAKLLEKPT-ALEFDVLKTSISRMGFGVQPISDKVAQEQQYVADAFYAQK-LIP-NKLTIQEAILKLK 3QSL Chain:A ((30-334)) ---------------------------------LEKAKVQIAVGGKPLIYYLPLTIAEVKGFFK----DEGLDVSIADFAGGSKALQAVVGGSADVVSGAFEHTLSLQAKGQFYRAFALQG-RAPMIGVGVSKKNLPGYKGPADLKGRKIGVTAPGSSTNMVVNFFLAKHGLKASDVSFIGVGAGAGAVTALRSGQIDAISNTDPVVSMLETSGDIQIIVDTRTLKDTKEIFGGNMPAGCLYAPQAFVDANPNTAQALTNAIVRADKWIQKAGADEIAKAVPEGYLLGDPAVYKAAIGKSMEGLSPDGVIPEDGAATALKALAAFVPDFDAAKVDPAKAWTNE-

General information: TITO was launched using: RESULT: Template: 3QSL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1799 -356 -0.20 -1.26 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -0.20 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_3QSL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QSL-query.scw PDB file : Tito_Scwrl_3QSL.pdb: