Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCCFVVYGGVLNMSDKKTSLVICFTPLQMLIAEKIVAQQPSVDLIVIALNNNDKYKYYYNIFQHPNIAKQYYLFDNNKSRFFKFINIIKFNENFKLEKFYENIYISSIDNKYTHNILSKVNFNNLYTYDDGTANIVKNSIYFKQTFESKLKDIVLKLIGIRFNLNKIKKLSRKHYTIYKGIQNIVEQTEYVSIIEKKEEDRNIFIDQEIKIFLGQPLKDIDK---N---FDTLALKKLLV--MEEVNYHFKHPRETDE--A-FFEEIKTSYIFEDFFAKELSKYRKVIIYTLSSTAALNIISLDNVEIRLIKNNTVEIKYPDLVQLFIKSGATVIDMDSIK 5FA1 Chain:B ((220-328)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------THSAAGVVLQVEDDSNLIAYNHDFTNISLLSYVRQRYEKEDILVRAHPGSLFRLRDDVFTID-DSANSLAFIN------QCNEVFTINSSVGLEAIL-TGKKTTVL------------------------------

General information: TITO was launched using: RESULT: Template: 5FA1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 361 3228 8.94 37.10 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.52 3D Compatibility (PKB) : 8.94 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_5FA1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FA1-query.scw PDB file : Tito_Scwrl_5FA1.pdb: