Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTNLAHKQEEDNVITLHPSTAKKKERTAMSDKFDKGYVMSSRLYRNEVKPFLGDAARNVYAELEEYISGFNKESDFVSYSQLQGKKIEGLEEHVR----KLSTATVRAGLKQLIEYGVISIVATNPKLGNKYKLNEISLVEHFSNKSTSETKALQKLNSTTLETKAQGTLETKDTIDIIYRYLIIDNLFNSLRSNKPLENHFFVYQENKKQVFLEQQKLEAEEKAKAEKERKDKVRKLSFDEVIKLTKTTFANLCDLELWEQYVAFRSQKANTKLTKNALNVIYKDLKAWGFQGSNQSLKTAISGNYLGLFAPKQQNHGFVNQSQASTRMSEIQELIAKEEAGNEQYGF 2P7C Chain:B ((6-73)) ---------------------------------------------------QISDAELEVMKVIWKHS--------SINTNEVIKE---------LSKTSTWSPKTIQTMLLRLIKKGALNHHKEG--RVFVYTPNID--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2P7C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 198 -11158 -56.35 -174.34 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -56.35 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.791

(partial model without unconserved sides chains): PDB file : Tito_2P7C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P7C-query.scw PDB file : Tito_Scwrl_2P7C.pdb: