Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MLLWLFEQLAGYHSSFQVVRYLTLRSLLSVLTSLTIGLVLGPIMIRKLQALK-YGQAVSSFAPENHAKKMGTPTMGGILILLSIGI-ST-LLWADLSNPYVWIVLGVMVVFGAVGWADDWIKIRYKDNAGLPARKKFFWTSVASLGAGIALYLIATQQSNAEYTANMLDLLIPFFKNLSIPLSIVPLGLAFIVFTYLVINGASNAVNLTDGLDGLAIMPVVMVATGLGVFAYLSGDIRFANYLHIPYVKYTSELVVICSAMIGAGLAFLWYNAHPAQVFMGDVGALA--LGAMLGTIAVMVRQEIVFAIMGGVFVMEAVSVFLQIGSLRMRNKRVFLMAPLHHHYEKQGWKE-TQVVIRFWIITIMLVVLGLMTLKLR 3DF7 Chain:A ((1-305)) SLKLFLFEFATCGER---------------------------IEDSTAVEGLAMFKSAFDGFKNYYEITGFVRPEFSC-LFTLPVDSMDSMEKYLEKSD-------AFLIIAPEDDF------LLYTLTKKAEKYCENLGSSSRAIAVT----------SDKWELYKKLRGEVQ-----VPQTSLRPLDCKFIIKPRTACIGFSDEVPDG------HIAQEFIEGINLSVSLAVGEDVKCLSVNEQIINNFRYAGAVVPARI----------SDEVKREVVEEAVRAVECVEGLNGYVGVDIVYS------DQPYVIEINARLTTPVVAFSRAYGASVADLLAGGEVKHVRRQMVRKSKSAEKPYVSVGDYTLEIIDLD

General information: TITO was launched using: RESULT: Template: 3DF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1710 -78775 -46.07 -267.94 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -46.07 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.188

(partial model without unconserved sides chains): PDB file : Tito_3DF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DF7-query.scw PDB file : Tito_Scwrl_3DF7.pdb: