Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMSPSSSGHVNMMKGQPFHVKYREIIRRMSFLIGALLVFRLGAHIPVPGINNAALENLFHANQGTILGLFNMFSGGALERMSILALGIMPYISASIIVQLMST-VIPSLEALKKEGEQGKRKINQYTRQGTLLLAVVQAVGMCAGLIGQ---GITLSSGLAFYIPAVTSLVAGTMFLMWLGEQITERGIGNGISMIIFAGIVAGLPKLIMQSVSSVDNGQTSLIGLVIFGLLSLGVLAAIVFIEKAQRRIPVNYAQKQQGRRIFTAQQTHLPLKINMAGVIPAIFASSLLLFPASLGQWVGSADPNAGLIKRSLQDLALVLSPGQPLYLVLFGALIIFFCYFYTALVFSPKEVSENLKRSGAYVPGIRPGEQTARYLDHILNRLTFIGAIYIAVICLMPMILQSSFGIP--FYLGGTSLLIVVVVVMDFMAQLQAHLTFHQYDNQTLMRKTTAHPKG 5EUL Chain:Y ((13-422)) ------------------------DIRNKIIFTLLMLIVFRIGTFIPVPSVNTDVLKLQ---DQLNAFGVLNIFCGGALQNFSIFAMGVMPYITASIIVQLLQMDVVPKFAEWSKQGEMGRRKLAQFTRYFTIVLGFIQALGMSYGFNNLAGGMLIQNP-IGTYLLIAVVLTAGTAFLMWLGEQITAKGVGNGISIIIFAGIVSGIPTILNQIYA--------IVRLLLVALAVVAVIVGVIYIQQAFRKIPIQ--------------STHLPLKVN-----PVIFAVSFLIAPPTIASFFGTND-------------RRTFDYTHPVGMTIYVVLIIAFTYFYAFVQVNPEQMADNLKKQGGYIPGIRPGKNTQEYVTRILYRLTLVGSLFLAFIAVLPVFFVNFANLPPSAQIGGTSLLIVVGVALETMKQLESQLVKRHYRGF------------

General information: TITO was launched using: RESULT: Template: 5EUL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Y - contact count / total energy / energy per contact / energy per residue : 1471 -206673 -140.50 -555.57 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain Y : 0.80 3D Compatibility (PKB) : -140.50 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_5EUL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EUL-query.scw PDB file : Tito_Scwrl_5EUL.pdb: