Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIREILLADTHNYYGILDERFIDLAHQFSRLQDA-RTGQGGAALAVYFRGQKVVDIYTGLKSQTEAWQPDTLAVCYSTGKGVLATLAHILVSEGFLEYDKPIATYWPEFAQNGKEQMTLRHVLSHQSGMFDVRNIIESAREMLDWSHMLVVVAATKPRFLAGEGNAYQALTFGW-LVGGVLEKATGQSLDQLMQNYLVEPLQLDGAYFGTPANELDRVARLIIQPKPEKP-ASTQVEKPKKPQTRKSSLSEKVITWTGQDPQDFQDAMIPKGMKNFSFFSDEGLQAVIPAANGTFTANSLAKIYAMLANQG--EW-DGQQLIRPEVFKELSTIQSYARDRVMPIPMNWRLGYHRIITM-GKRAKNGFGHIGYNGSGAWCDPERDLSFAYTHNLQIGSITGDYRLWGLTQEALRCTDQILKGRKGWF 3ZYT Chain:A ((4-370)) --------------QVIAPGFEPVAELFGVFLEQDP--DYSAQVAAYHRGVKVLDLSGG-----PHIRPDSVTGVFSCSKGMAGLVMALLVQDGELDLEAEVVKYWPEFGVEGKSSITVAQLLSHRAGLLGVE-GGLTLHEVNNSELAAAKLAELPPLWKPGTAFGYHALTI-GIFMEELCRRITGSTLQEVFEQRIRAVTGANFYL-GLPESEESRFAQFRWA--ADPSWPWVDPASHF-------------G-LAANAA---V----GDILD--LPNIREVRAAGLSSAAGVASAEGMARIYAAALTGLEGKSAMP-PLLTEETIRTVSAEQVFGIDRVFGETGCFGTVFMKSHTRMPFGSYRAFGHDGASASLGFADPVYELGFGYVPQTAEPGGV-GCRNFQLSSAVREVIAGF--------

General information: TITO was launched using: RESULT: Template: 3ZYT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2286 -67281 -29.43 -186.89 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -29.43 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_3ZYT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZYT-query.scw PDB file : Tito_Scwrl_3ZYT.pdb: