Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLLIASLIAISSSSIFAADTTASAAKNDVFKQAEQLYAAKNYPAAFQEVQRLAQTG--NAQAIYNLGYMTQMGQGTAKDNAKALKYYEDASNKGY--AQASYTLAQIYETGELGVAKDSNKFSQYIQKAAAQGS-----------------------------DDATVKIATILFAQKKPQSHQIALQKLAPLIRKGNYPAI---QVKALYDISQGVENKNPLMKRQGIEALQTIAQKG--YAPASMALATMMANGNIIPQNLPQAKQIFTELAKQN---VPNARESLASVEKIIAEKNKQAAAAPTQPAPKK 4EQF Chain:A ((64-345)) --------------------------DWPGAFEEGLKRLKEGDLPVTILFMEAAILQDPGDAEAWQFLGITQAE----NENEQAAIVALQRCLELQPNNLKALMALAVSYTN-----TSHQQDACEALKNWIKQNPKYKYLDSSVLEGVKELYLEAAHQNGDMIDPDLQTGLGVLFHLSG---EFNRAIDAFNAALTVRPEDYSLWNRLGATLANGD--------RSEEAVEAYTRALEIQPGFIRSRYNLGISCINLG----AYREAVSNFLTALSLQRKS-GNIWAALRIALSLMDQPEL-------------

General information: TITO was launched using: RESULT: Template: 4EQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1066 -5048 -4.74 -24.04 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -4.74 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_4EQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EQF-query.scw PDB file : Tito_Scwrl_4EQF.pdb: