Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPGTPMTVIPTIDIVDALAAEYAAKSPREILELALSQQG-EIAISFSGAEDVVLIDMASRLGKPFRVFSLDTGRLHPETYQFFETVRKHYNINIEICFPDAEAVQSMVNEKGLFSFFKDGHQECCGIRKVQPLRKKLATLDGWITGQRKDQSPGTRTEIPVVQADAGFSGPGKQLIKYNPLANWSSADVWSYIRMMEIPYNPLHERGFVSIGCEPCTRPVLPNQHEREGRWWWEEATQKECGLHAGNLKK 2GOY Chain:F ((32-249)) ----------------ALASSLADKSPQDILKAAFEHFGDELWISFSGAEDVVLVDMAWKLNRNVKVFSLDTGRLHPETYRFIDQVREHYGIAIDVLSPDPRLLEPLVKEKGLFSFYRDGHGECCGIRKIEPLKRKLAGVRAWATGQRRDQSPGTRSQVAVLEIDGAFSTPEKPLYKFNPLSSMTSEEVWGYIRMLELPYNSLHERGYISIGCEPCTRPVLPNQHEREGRWWWE----------------

General information: TITO was launched using: RESULT: Template: 2GOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1047 -3353 -3.20 -15.45 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain F : 0.89 3D Compatibility (PKB) : -3.20 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_2GOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GOY-query.scw PDB file : Tito_Scwrl_2GOY.pdb: