Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMRIGLFLLTNLAVLVVAGIILSLFGVGSYHGAGGLNLGNLLVICFVF--GMVGSL-----VSL----------FMSKWMAKKTTGTELIDPNAPRNQAESWLLQTVAELSQRAGINMPEVG---IFPSYQSNAFATGWN-----KNDALVAVSSGLLERMNKDEL--------------------------------RAVLAHEIGH---VANGDMVTLALIQGVVNAFVMFFAR--------VVGDFIDRNVFGRQD--------NEAPGMGYFIITMVLDIV-FGILASAIVMWFSRYREYRADEAGARLAGKQAMIS-----ALLRLQAETELPDQMPKE------MKAFAIAEGKEQGFSLAALFQTHP--------TIEQRV-AALHQLDCP----------------------- 4Q6O Chain:A ((21-437)) MIEKAP---TNVEDRDKAPHLLLLAGIQG-DEPGGFNATNLFLMHYSVLKGLVEVVPVLNKPSMLRNHRGLYGDMNRKFAALDKNDPEYPTIQEIKSLIAKPSIDAVLHLHDGGGYYRPVYVDAMLNPKRWGNCFIIDQDEVKGAKFPNLLAFANNTIESINAHLLHPIEEYHLKNTRTAQGDTEMQKALTFYAINQKKSAFANEASKELPLASRVFYHLQAIEGLLNQLNIPFKRDFDLNPNSVHALINDKNLWAKISSLPKMPLFNLRPKLNHFPLPHNTKIPQIPIESNAYIVGLVKNKQEVFLKYGNKLMTRLSPFYIEFDPSLEEVKMQIDNKDQMVKIGSVVEVKESFYIHAMDNIRANVIGFSVSNENKPNEAGYTIKFKDFQKRFSLDKQERIYRIEFYKNNAFSGMILVKFV

General information: TITO was launched using: RESULT: Template: 4Q6O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1614 -60752 -37.64 -204.55 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -37.64 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.172

(partial model without unconserved sides chains): PDB file : Tito_4Q6O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q6O-query.scw PDB file : Tito_Scwrl_4Q6O.pdb: