Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIVILNKPYDVLSQFRKDEAHMTVSDFVD--------DPTLRIAGRLDMDSEGLVFLTDHGGLNQFITNPA--NKKYKTYLVQVDGDVTEEALEQLRKGVELNDGMTLPAKAIKVSEPEWLWDRDPPVRYRASIPTSWIEISICEGRNRQVRRMTSAVGFPTLRLIRTKIGTIDLVQLGLQPGETKEIEPLLYPDFKDVPAEQPYRSRSYVRKPGGTGGKPMVRKNKDGSAKKSGTKRIWQMDESEKPRRKTNGTTRPNTKAPRGRSRNSR 1PRZ Chain:A ((92-258)) -DIIVINKPRDLVVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVESLQRREITREYEAVAIGHMTAG--GTVDEPISRHPTK--RTHMAVHPMGKPAVTHYRIMEH--FRVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPR--------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1PRZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 690 -2886 -4.18 -18.38 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -4.18 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_1PRZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PRZ-query.scw PDB file : Tito_Scwrl_1PRZ.pdb: