Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSILSWDDFEDDSQKPAAPEHKSAPVNPEKTPDSQNVSAAQPSSQSMAAPQSTGTHSVRSTNPTDSMARASEALEHLDVAPGLEELEMGAQRVQVDDKAMINC-RADLNQLVPFKYEWAWQKYLDGCANHWMPQEVNMNHDIALWKSENGLTEDERTIVMRSLGFFSTADSLVANNLVLAIYRHITNPECRQYILRQAFEEAIHTHAYQYCIESLGMDEGEVFNMYREIPSVARKAAWGLKYTQSLSDPTFHTGTPENDQRLLRNLIAFYCVLEGIFFYCGFTQILSMGRRNKMNGVAEQFQYILRDESMHLNFGIDMINQIKIENPHLWTAEFQQEVIQMILEGTMLEIEYARDTMPRGVLGMNASMMEEYLKFICNRRLSQLGLPEQFAGVTNPFAWMSEMMDLRKEKNFFETRVTDYQTGGALSW 4M1I Chain:B ((26-340)) -------------------------------------------------------------------------------------LDGKQKRVNLNSKRLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEIPMGKDIELWKSDR-LSEDERRVILLNLGFFSTAESLVGNNIVLAIFKHVTNPEARQYLLRQAFEEAVHTHTFLYICESLGLDEKEIFNAYNERAAIKAKDDFQMEITGKVLDPNFRTDSVEGLQEFVKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMIGIGEQYQYILRDETIHLNFGIDLINGIKEENPEIWTPELQQEIVELIKRAVDLEIEYAQDCLPRGILGLRASMFIDYVQHIADRRLERIGLKPIYH-TKNPFPWMSE--------------------------

General information: TITO was launched using: RESULT: Template: 4M1I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1507 -66733 -44.28 -212.52 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -44.28 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_4M1I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M1I-query.scw PDB file : Tito_Scwrl_4M1I.pdb: