Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLNMPEPTSTTAATTTSLAAVSL------LPFI------NGNALLGAVLGAAFVA-YLEKDLSAKQRIVFMLLCVGFGY--------LLGPEITSRTQYISSDATASMVAAIFAIYILIKLLDWVKNSTLAQLWKTFRGGGNS- 1EKR Chain:A ((11-156)) GEAHMVDVSAKAETVREARAEAFVTMRSETLAMIIDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETLCRLTGKTGVEMEALTAASVAALTIYDMCKAVQ--KDMVIGPVRLLAKSSGDFK

General information: TITO was launched using: RESULT: Template: 1EKR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 548 -24725 -45.12 -204.34 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -45.12 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_1EKR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EKR-query.scw PDB file : Tito_Scwrl_1EKR.pdb: