Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGMEEHLLTQKLQQRLRFSTHIQEAQAAVLIAITNENNPKVLLTRRSIHMNNHAGEVSFPGGKRDPSDTSNIVVALREAQEETALNPFDVQLLGDLPMQRA-RSGLSVKPIVGLIPPEVTLI-PQPTEIDRIFFVPLQQLIEMRPTPYEVRYAHQSLYFPSLQIDNEIIWGLTAQMLIALFKYGLGYQKEWPFLLNAPTFGMPKFSHKK 1SJY Chain:A ((11-157)) ------------------------VELRAAGVVLLNE-RGDILLVQEKGI--EKAGLWHIPSGAVEDGEN-PQDAAVREACEETGLRVRPVKFLGAYLGRFPDGVLILRHVWLAEPEPGQTLAPAFTDEIAEASFVSREDFAQLYAAGQI-R----MYQTKLFYADALREKGFPAL----------------------------------

General information: TITO was launched using: RESULT: Template: 1SJY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 683 -35213 -51.56 -249.73 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -51.56 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_1SJY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SJY-query.scw PDB file : Tito_Scwrl_1SJY.pdb: