Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYQINALNPKDEGHKARKRFGQNFLHDQRVIAKIVRSVNPRTGDNIVEIGPGLAALTSPLIGECDALTVVELDRDL-------AAGLPERVPHPERLTIVEADALKYDFSQLVKDGRPLRVVGNLPYNISTPLLFHLLEFGSQVKDMHFMLQKEVVERITAEPNTKEYGRLSVMIQYYCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYEQKPITAKDEKALARLVAHVFTQRRKTLRNSLKG-----MLAEDGFEKAGVDPMARPETLTLAEFVALADQMVA 3FUX Chain:B ((18-267)) ------------GLFADKRFGQNFLVSEAHLRRIVEAARPFTGP-VFEVGPGLGALTRALLEAGAEVTAIEKDLRLRPVLEETLSGLPVR--------LVFQDALLYPWEE-VPQGSLL--VANLPYHIATPLVTRLLKTG-RFARLVFLVQKEVAERMTARPKTPAYGVLTLRVAHHAVAERLFDLPPGAFFPPPKVWSSLVRLT-----PTGALDDPGLFRLVEAAFGKRRKTLLNALAAAGYPKARVEEALRALGLPPRVRAEELDLEAFRRLREGL--

General information: TITO was launched using: RESULT: Template: 3FUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1189 -29112 -24.48 -122.32 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -24.48 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_3FUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FUX-query.scw PDB file : Tito_Scwrl_3FUX.pdb: