Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---PWNNYCQ----LTLMRGRQIQAQSYLVRRSFPLTT-VV----------IRLEPGKSR--------------SFKLNWNCRLK---IPRGV----------------VPEGHWLYADMKYMTPSMAADLNNLLDGAPWPLEWPLDRESLRLESQRNSRVFQS- 1G13 Chain:A ((1-162)) SSFSWDN-CDEGKDPAVIRSLTLEPDPIIVPGNVTLSVMGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDL--ELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

General information: TITO was launched using: RESULT: Template: 1G13.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 266 -1475 -5.55 -13.41 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -5.55 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.65 QMean score : -0.017

(partial model without unconserved sides chains): PDB file : Tito_1G13.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G13-query.scw PDB file : Tito_Scwrl_1G13.pdb: