Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NCTANILN-I-NEVVIATGCVPAGG---NLIIRVGSDHSYLIRATVSCGLSLNPSQSFINGESLAS-GGRC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 4M0M Chain:A ((13-740)) EQKVKTVTLSFLGTGQHREKVHHILTSFHNTISEVNKDNPTVAMRMFDGPGSEPKSGDSKDPIPGTYIYNPKDNSKILISPVISQTITNAIQKLTGNLAGEGIEHLLFEAVLYLNDIIEKNGGKLPETVNLHGFSRGADTCMRMANLLYQLYPDIKVNLFLIDQVPGPGKRDDPHSYTVPPNVEHFESTLMLHEYRPGFDPQHSGRYVIADPEKTKVVVKPYYGEHNTGNRVTEDPNTNHTAILLNDDMNRFCRETGSLPSVGISPPIIARVGDKKEEVRTHS-----ELSPEKRF---ELLCGMKENEWGYAKLTKKYHERSILSKREDYVQDSRLFVNQEHRELFKQLYPKSFNWFFEKNHGGQTKKEEVIVELKSLSEDPRYEHFFSSLAKHFQINENNIAGTLPEPSGIDRDEKSSFGQPPVRDRLSYLQHSLTSIANYYHYHCDEKSSTNESVKNLLLERVKESRTKPDSEAIKHLEQTMDEVRQILESKNEKGFLWQQINHISPNARQYCEQVKAALREHLEHNQVLSDTQKEEIRKAMDRMDNIVNDSSKDSQQKYREIRREVIELNAKATTPEDDNQLTRSHFQKAYFEGDTQKTLNLESLSQTLNQLSKAHYGETSMTDKITQRLDGYKNRNWFWNSVKEVLNFFNIPLPKLHSEVKEQIADKLKERLVDLKEKGMGNDVNAITRELGKAREDLIEHYKKTGELDKIINKSMEELLVARK

General information: TITO was launched using: RESULT: Template: 4M0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 154 6172 40.08 108.28 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 40.08 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.213

(partial model without unconserved sides chains): PDB file : Tito_4M0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M0M-query.scw PDB file : Tito_Scwrl_4M0M.pdb: