Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSWKNWLIKINYCVYKKKRKGRMRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIYFREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFIAADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEGREFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQDEKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKEDNKEYSLKDLITKVSKESDNVAHNLLGYYIS-NQSDATFKSKMSAIMGDDW------------DPKEKLISSKMAGKFMEAIYNQN-------GFVLESLTKTDFDSQRIAKGVS--VKVAHKIGDAD-EFKHDTGVVYAD-S-PFILSIFTKNSDY------DTISKIAKDVYEVLK 1YLT Chain:A ((5-263)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQQKLAALEKSS--GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKPAD-------LVNYNPIAEK-HVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIG-DETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNTTGAASIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESRRDVLASAARIIAEGL-

General information: TITO was launched using: RESULT: Template: 1YLT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1197 -1459 -1.22 -6.40 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -1.22 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_1YLT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YLT-query.scw PDB file : Tito_Scwrl_1YLT.pdb: