Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQVNIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRKAR-AREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERRGMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGKTCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVLDTIYLPEERL-IDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI 3DAH Chain:C ((7-313)) ----GLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQSTCAPTNDNLMELMIMVDALKRASAGRITAAIPYFGYARQDRRPRSARVAISAKVVANMLEIAGVERIITMDLHADQIQGFFDIPVDNIYATPILLGDLRKQNY--PDLLVVSPDVGGVVRARALAKQLNCDLAIIDKRR---------VMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKERGAKQVFAYATHPVLSGGAADRIAASALDELVVTDTIPLSAESLACPKIRALSSAGLLAETFSRIRRGDSVM---------

General information: TITO was launched using: RESULT: Template: 3DAH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1653 -95097 -57.53 -319.12 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.87 3D Compatibility (PKB) : -57.53 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.585

(partial model without unconserved sides chains): PDB file : Tito_3DAH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DAH-query.scw PDB file : Tito_Scwrl_3DAH.pdb: