Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIKQYLTATE---RVSIIHTDEKIDSDD---EPT------RAIRNK---------KNASMVLAAKAVKDGEADAVLSAG-NTGALLAAGFFIVGRIKNIDRP-----GLMSTLPTVDGKGFDMLD-LGAN-----A-ENTAQHLHQYAVLGSFYAKNVRGIAQPRVGLLNNGT----E---SS-KGDPLRKETYELLAADESL-NFIGNVEARDLMN----GVADVVVA-DGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLNYSDVGGAVLFGVKAPVVKTHGSSDA------------KAVYSTIRQIRTMLETDVVAQTAREFSGE 4JQP Chain:B ((29-341)) -------------GAEVVVKSLAHASVYA-QCRPLVIGDAKRLERANQIVGGEMKIRRIEDASEARYEQGTIDCIDLGLIPDDLPFGQLSAIAGDAAYQYIKRAVELAQSGKIDAICTAPLNKEALHAGGHKYPGHTEMLAHLTGVDEVSMMLVAPQ-LRVIHVTTHIGIIDAIRKIEPGLVQRTIERGN---ATLVK-AGIERPRIGVCGINPHAGENGLFGYGEEEEKIIPAVTLLQER--GLDVTGPLPADTLFFRAGRGDFDLVVAMYHDQGHGPVKVLG-------------L------------------------------EAGVNVTVGLEVIRTSVDHGTAFDIAGKGVVDEGSMLEALRQGAELATR-------------

General information: TITO was launched using: RESULT: Template: 4JQP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1130 13768 12.18 54.42 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 12.18 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_4JQP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JQP-query.scw PDB file : Tito_Scwrl_4JQP.pdb: