Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLE--------VEVSSLGVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRL-------------------IN-LPLLYLGTLMVGASVAVINVLLPSLIQANQP-KKIGFLTTLYVTSMGIATALASYLAVPITQA--------SSWKGLILLLTLLCLATFLVWLPNHRYNHRL--APQ-TK-----------------QKS----QIKVMRNKQVWA----------IIIFSGFQSLIFYTVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVSLAGVIGISMLFF---P-INNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGLSQTGGYILAAFGPTLFGYSF------DLFHSWVPSVAALLLIDILMTVALFTVDRADKIL 4GBY Chain:A ((9-458)) ---YIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVAPQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFG-RKP-LQIIGA-LGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIF-PNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFH-NGFSYWIYGCMGVLAALF-----------

General information: TITO was launched using: RESULT: Template: 4GBY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1553 -218642 -140.79 -592.52 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -140.79 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_4GBY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GBY-query.scw PDB file : Tito_Scwrl_4GBY.pdb: